![]() ![]() ![]() ![]() |
![]() ![]() |
![]() ![]() ![]() Главная » Наука » Публикации » |
![]() |
![]() |
RNA. 2008 Apr;14(4):717-35. PubMed; doi 10.1261/rna.819308 Comparative genomic analysis of T-box regulatory systems in bacteria.Vitreschak, A. G.; Mironov, A. A.; Lyubetsky, V. A.; Gelfand, M. S.T-box antitermination is one of the main mechanisms of regulation of genes involved in amino acid metabolism in Gram-positive bacteria. T-box regulatory sites consist of conserved sequence and RNA secondary structure elements. Using a set of known T-box sites, we constructed the common pattern and used it to scan available bacterial genomes. New T-boxes were found in various Gram-positive bacteria, some Gram-negative bacteria (delta-proteobacteria), and some other bacterial groups (Deinococcales/Thermales, Chloroflexi, Dictyoglomi). The majority of T-box-regulated genes encode aminoacyl-tRNA synthetases. Two other groups of T-box-regulated genes are amino acid biosynthetic genes and transporters, as well as genes with unknown function. Analysis of candidate T-box sites resulted in new functional annotations. We assigned the amino acid specificity to a large number of candidate amino acid transporters and a possible function to amino acid biosynthesis genes. We then studied the evolution of the T-boxes. Analysis of the constructed phylogenetic trees demonstrated that in addition to the normal evolution consistent with the evolution of regulated genes, T-boxes may be duplicated, transferred to other genes, and change specificity. We observed several cases of recent T-box regulon expansion following the loss of a previously existing regulatory system, in particular, arginine regulon in Clostridium difficile and methionine regulon in Lactobacillaceae. Finally, we described a new structural class of T-boxes containing duplicated terminator-antiterminator elements and unusual reduced T-boxes regulating initiation of translation in the Actinobacteria. ![]() |
![]() |
![]() ![]() ![]() ![]() |
Почтовый адрес: 119991 г. Москва, ГСП-1, Ленинские горы МГУ 1, стр. 73, Факультет биоинженерии и биоинформатики, комната 433. Телефон / факс: Справочная телефонов МГУ E-mail: bioeng@genebee.msu.ru © 2011 Факультет биоинженерии и биоинформатики Московского Государственного Университета имени М.В.Ломоносова
|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |