Главная страница Карта сайта Контактная информация

Главная » Наука » Публикации »


Dev Cell. 2009 Sep;17(3):323-33. PubMed; doi 10.1016/j.devcel.2009.07.010

CLIP-170-dependent capture of membrane organelles by microtubules initiates minus-end directed transport.

Lomakin, A. J.; Semenova, I.; Zaliapin, I.; Kraikivski, P.; Nadezhdina, E.; Slepchenko, B. M.; Akhmanova, A.; Rodionov, V.

Cytoplasmic microtubules (MTs) continuously grow and shorten at free plus ends. During mitosis, this dynamic behavior allows MTs to capture chromosomes to initiate their movement to the spindle poles; however, the role of MT dynamics in capturing organelles for transport in interphase cells has not been demonstrated. Here we use Xenopus melanophores to test the hypothesis that MT dynamics significantly contribute to the efficiency of MT minus-end directed transport of membrane organelles. We demonstrate that initiation of transport of membrane-bounded melanosomes (pigment granules) to the cell center involves their capture by MT plus ends, and that inhibition of MT dynamics or loss of the MT plus-end tracking protein CLIP-170 from MT tips dramatically inhibits pigment aggregation. We conclude that MT dynamics are required for the initiation of MT transport of membrane organelles in interphase cells, and that +TIPs such as CLIP-170 play an important role in this process.


  Московский Государственный Университет имени М.В.Ломоносова

Почтовый адрес:
119991 г. Москва, ГСП-1, Ленинские горы МГУ 1, стр. 73,
Факультет биоинженерии и биоинформатики, комната 433.

Телефон / факс: +7 (495) 939-41-95
Справочная телефонов МГУ +7 (495) 939-10-00

E-mail: bioeng@genebee.msu.ru

© 2011 Факультет биоинженерии и биоинформатики
Московского Государственного Университета имени М.В.Ломоносова


- создание сайта, 2010