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J Mol Biol. 2012 Feb 24;416(3):389-99. PubMed; doi 10.1016/j.jmb.2012.01.002

Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32.

Pavlova, O.; Lavysh, D.; Klimuk, E.; Djordjevic, M.; Ravcheev, D. A.; Gelfand, M. S.; Severinov, K.; Akulenko, N.

Escherichia coli phage phiEco32 encodes two proteins that bind to host RNA polymerase (RNAP): gp79, a novel protein, and gp36, a distant homolog of sigma(70) family proteins. Here, we investigated the temporal pattern of phiEco32 and host gene expression during infection. Host transcription shutoff and three distinct bacteriophage temporal gene classes (early, middle, and late) were revealed. A combination of bioinformatic and biochemical approaches allowed identification of phage promoters recognized by a host RNAP holoenzyme containing the sigma(70) factor. These promoters are located upstream of early phage genes. A combination of macroarray data, primer extension, and in vitro transcription analyses allowed identification of six promoters recognized by an RNAP holoenzyme containing gp36. These promoters are characterized by a single-consensus element tAATGTAtA and are located upstream of the middle and late phage genes. Curiously, gp79, an inhibitor of host and early phage transcription by sigma(70) holoenzyme, activated transcription by the gp36 holoenzyme in vitro.



 


  Московский Государственный Университет имени М.В.Ломоносова



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Московского Государственного Университета имени М.В.Ломоносова


 





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